Zorbax 300Extend-C18
Zorbax 300Extend-C18 HPLC Columns from Agilent Technologies
Zorbax 300Extend-C18 is a wide-pore HPLC column for high efficiency separations of peptides, polypeptides, and small proteins from pH 2-11.5. Extend-C18 and 300Extend-C18 columns incorporate a unique patented bidentate silane, combined with a double-endcapping process that protects the silica from dissolution at high pH. At high pH, the retention and selectivity of peptides can change dramatically as a result of changes in amino acid charge.
Excellent recoveries of hydrophobic polypeptides have been achieved at high pH with the 300Extend C18. In addition, LC-MS sensitivity of peptides can also be improved at high pH using a simple ammonium hydroxide mobile phase. These conditions allow for ions to be detected in both positive and negative ion modes simultaneously with the 300Extend-C18. The Zorbax 300Extend-C18 is a rugged column and is ideal for LC-MS with ammonium hydroxide mobile phase.
All pictures shown are for illustration purpose only. Actual product may vary.
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- Features
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- Rugged, high and low pH separations of peptides, polypeptides, and small proteins from pH 2 - 11.5
- Different selectivity possible at high and low pH
- High efficiency and good recovery of hydrophobic peptides at high pH
- Ideal for LC-MS with ammonium-hydroxide-modified mobile phase
- Specifications
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ZORBAX 300Extend-C18 Pore Size 300 Å Surface Area 45 m2/g Temp Limits 60 °C up to pH 8, 40 °C from pH 8 - 11.5 pH Range 2 - 11.5 Endcapped Double Carbon Load 4% - Literature
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Zorbax 300Extend-C18 Data Sheet
Data sheet for the Zorbax 300Extend-C18 columns
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Agilent Bio Column Selection Guide
Guidance on column selection for Biochromatography from Agilent
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Reversed Phase Analysis of Proteins
Protein Identification and Impurity Profiling using Reversed-Phase HPLC/UHPLC. Agilent biocolumns can boost your accuracy and productivity. This document covers complete portfolio of reversed-phase columns for protein identity applications.
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